Nanobody cluster imaging probe based on sortase and transglutaminase
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Abstract
Sortase and transglutaminase was rendered for preparing a targeted protein cluster probe, which is composed nanobody and green fluorescence protein (sfGFP). A unique substrate, which can be recognized by both sortase and transglutaminase, was designed for nanobody and sfGFP conjunction by solid phase synthesis. The targeted protein cluster probe, namely KK-sfGFP(Nb), can specificly bind to the EGFR overexpressed cells and shows green fluorescence as signal. The KK-sfGFP(Nb) exhibits fast accumulation in mouse xenograft tumor and the fluorescence signal gradually metabolizes within 9 h.
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