ISSN 0253-2778

CN 34-1054/N

open
Open AccessOpen Access JUSTC

Localization and characterization of Fe(Ⅲ) reductase of Desulfovibrio dechloracetivorans strain SF3

Cite this: JUSTC, 2009, 39(4): 337-343
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  • Author Bio:

    WANG Jia-chen, female, born in 1983, master. Research field: dissimilately reductase of anaerobic bacteria.

  • Corresponding author:

    SUN Bao-lin

  • Received Date: March 20, 2008
  • Revised Date: April 20, 2008
  • Published Date: April 29, 2009
  • Both physiological and biochemical studies have demonstrate that Fe(Ⅲ)-EDTA is a favorite soluble Fe(Ⅲ) of strain SF3. The UV-visible spectra of dithionite-reduced, soluble Fe(Ⅲ) reoxidized cell extracts indicates the involvement of c-type cytochromes in Fe(Ⅲ) reduction. Fractionation experiments suggest that 87% of the Fe(Ⅲ) reducing activity is localized at soluble fraction, 74% of the Fe(Ⅲ)-reducing activity is found in the spheroplasts, which may indicate a distinct mechanism for Fe(Ⅲ) reduction. Fe(Ⅲ) reductase of strain SF3 exhibits the highest activity at 25 ℃, pH 74, and shows a great oxygen-tolerance ability.

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