Hefei National Laboratory for Physical Sciences at Microscale, and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China
Both physiological and biochemical studies have demonstrate that Fe(Ⅲ)-EDTA is a favorite soluble Fe(Ⅲ) of strain SF3. The UV-visible spectra of dithionite-reduced, soluble Fe(Ⅲ) reoxidized cell extracts indicates the involvement of c-type cytochromes in Fe(Ⅲ) reduction. Fractionation experiments suggest that 87% of the Fe(Ⅲ) reducing activity is localized at soluble fraction, 74% of the Fe(Ⅲ)-reducing activity is found in the spheroplasts, which may indicate a distinct mechanism for Fe(Ⅲ) reduction. Fe(Ⅲ) reductase of strain SF3 exhibits the highest activity at 25 ℃, pH 74, and shows a great oxygen-tolerance ability.
Abstract
Both physiological and biochemical studies have demonstrate that Fe(Ⅲ)-EDTA is a favorite soluble Fe(Ⅲ) of strain SF3. The UV-visible spectra of dithionite-reduced, soluble Fe(Ⅲ) reoxidized cell extracts indicates the involvement of c-type cytochromes in Fe(Ⅲ) reduction. Fractionation experiments suggest that 87% of the Fe(Ⅲ) reducing activity is localized at soluble fraction, 74% of the Fe(Ⅲ)-reducing activity is found in the spheroplasts, which may indicate a distinct mechanism for Fe(Ⅲ) reduction. Fe(Ⅲ) reductase of strain SF3 exhibits the highest activity at 25 ℃, pH 74, and shows a great oxygen-tolerance ability.