Zinc(II) coordinated lipid for effective and serum-tolerant gene delivery

Zhou Xiaohong; Elbayomi Smaher M; Nie Xuan; Shao Qi; Wang Haili; Wang Fei; You Yezi

doi:10.3969/j.issn.0253-2778.2020.11.003

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Open Access JUSTC Research Article

Zinc(II) coordinated lipid for effective and serum-tolerant gene delivery

1.
CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, China
2.
Neurosurgical Department, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230036, China

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https://doi.org/10.3969/j.issn.0253-2778.2020.11.003

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Author Bio:
Zhou Xiaohong is currently a Ph.D. student in the Lab for CAS Key Laboratary of Solf Matter Chemistry, Department of Polymer Science and Engineering under the supervision of Prof. You Yezi at University of Science and Technology of China. Her research mainly focuses on biomaterials.
Corresponding author: Wang Fei (corresponding author) received his Ph.D. degree in Capital Medical University. He is currently an assistant director physician at the First Affiliated Hospital of University of Science and Technology of China. E-mail: neurosurgeonahwf@126.com; You Yezi (corresponding author) received his Ph.D. degree in Chemistry and Physics of Polymer from University of Science and Technology of China. He is currently a professor at University of Science and Technology of China. His research interests include designing gene vectors for gene delivery and gene editing, designing anti-tumor nanomedicines, and designing polymers for anti-bacterial. E-mail: yzyou@ustc.edu.cn
Received Date: 27 October 2020
Rev Recd Date: 23 November 2020
Publish Date: 30 November 2020

Abstract Full text PDF

Abstract

Abstract

In recent years, non-viral vectors, such as cationic polymers and liposomes, have attracted increasing attentions for their applications in gene delivery. However, reduced transfection efficiency inhibits their further use in the presence of serum. Therefore, it is necessary to develop serum-tolerant non-viral gene vectors. Herein, a novel Zn coordinated lipid is synthesized, which self-assembles with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine lipid (DOPE), forming cationic liposome (Zn-DTAc liposome). The experimental results demonstrate that Zn-DTAc liposomes can effectively condense pDNA into nanoparticles, which have high cellular uptake and effective endosomal escape and exhibit efficient gene transfection even in the presence of 50% fetal bovine serum.

Abstract

In recent years, non-viral vectors, such as cationic polymers and liposomes, have attracted increasing attentions for their applications in gene delivery. However, reduced transfection efficiency inhibits their further use in the presence of serum. Therefore, it is necessary to develop serum-tolerant non-viral gene vectors. Herein, a novel Zn coordinated lipid is synthesized, which self-assembles with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine lipid (DOPE), forming cationic liposome (Zn-DTAc liposome). The experimental results demonstrate that Zn-DTAc liposomes can effectively condense pDNA into nanoparticles, which have high cellular uptake and effective endosomal escape and exhibit efficient gene transfection even in the presence of 50% fetal bovine serum.