ISSN 0253-2778

CN 34-1054/N

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Open AccessOpen Access JUSTC Original Paper

Effect of magnetic nanoparticle concentration and other conditions on ice crystal growth of VS55 solution during devitrification

  • 1.

    Department of Thermal Science and Energy Engineering, University of Science and Technology of China, Hefei 230026, China

  • 2.

    Institute of Bio-thermal Science and Technology, University of Shanghai for Science and Technology, Shanghai 200093, China

Cite this:
https://doi.org/10.3969/j.issn.0253-2778.2020.06.014
  • Received Date: 11 March 2020
  • Accepted Date: 27 June 2020
  • Rev Recd Date: 27 June 2020
  • Publish Date: 30 June 2020
    Abstract

  • Abstract

    The effects of the concentration of magnetic nanoparticles, isothermal temperature and the rate of cooling/rewarming on the crystallization behavior of VS55 during the devitrification process under isothermal scanning and continuous scanning methods were studied using a cryomicroscope system. The results show that: With the increase of nanoparticle concentration and isothermal temperature, the growth rate, initial size and crystal density of ice crystals increase generally, promoting the growth of ice crystals. Continuous scanning of VS55 with different magnetic nanoparticle concentration groups was performed, and the results were consistent with the isothermal scanning conclusions. The crystal devitrification growth of the magnetic nanoparticle-added solution was obvious. When the cooling rate is increased to 5 ℃ / min, the initial size of ice crystals and their number increase significantly and the growth rate also increases slightly. A cooling rate above 5 ℃ / min has little effect on the growth of ice crystals. As the heating rate increases, the ice crystal size, growth rate, and number of ice crystals decrease.

    Abstract

    The effects of the concentration of magnetic nanoparticles, isothermal temperature and the rate of cooling/rewarming on the crystallization behavior of VS55 during the devitrification process under isothermal scanning and continuous scanning methods were studied using a cryomicroscope system. The results show that: With the increase of nanoparticle concentration and isothermal temperature, the growth rate, initial size and crystal density of ice crystals increase generally, promoting the growth of ice crystals. Continuous scanning of VS55 with different magnetic nanoparticle concentration groups was performed, and the results were consistent with the isothermal scanning conclusions. The crystal devitrification growth of the magnetic nanoparticle-added solution was obvious. When the cooling rate is increased to 5 ℃ / min, the initial size of ice crystals and their number increase significantly and the growth rate also increases slightly. A cooling rate above 5 ℃ / min has little effect on the growth of ice crystals. As the heating rate increases, the ice crystal size, growth rate, and number of ice crystals decrease.
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    • References(24)
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    HOU Y, LU C, DOU M, et al, Soft liquid metal nanoparticles achieve reduced crystal nucleation and ultrarapid rewarming for human bone marrow stromal cell and blood vessel cryopreservation[J]. Acta Biomaterialia1, 2020, 102: 403-415.
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    LI W J, ZHOU X L, LIU B L, et al. Effect of nanoparticles on the survival and development of vitrified porcine GV oocytes[J]. Cryo Letters, 2016, 37(6):401-405.
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    PAN J J, SHU Z Q, ZHAO G, et al. Towards uniform and fast rewarming for cryopreservation with electromagnetic resonance cavity: Numerical simulation and experimental investigation[J]. Applied Thermal Engineering, 2018, 140:787-798.
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    RING H L, GAO Z, SHARMA A, et al. Imaging the distribution of iron oxide nanoparticles in hypothermic perfused tissues[J]. Magnetic Resonance in Medicine, 2020, 83(5):1750-1759.
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    WANG T, ZHAO G, DENG Z S, et al. Theoretical investigation of a novel microwave antenna aided cryovial for rapid and uniform rewarming of frozen cryoprotective agent solutions[J]. Applied Thermal Engineering, 2015, 89:968-977.
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    WANG T, ZHAO G, LIANG X M, et al. Numerical simulation of the effect of superparamagnetic nanoparticles on microwave rewarming of cryopreserved tissues[J]. Cryobiology, 2014, 68(2):234-243.
    [14]
    ETHERIDGE M L, XU Y, ROTT L, et al. RF heating of magnetic nanoparticles improves the thawing of cryopreserved biomaterials[J]. Technology, 2014, 2(3):229-242.
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    WANG J, ZHAO G, ZHANG Z, et al. Magnetic induction heating of superparamagnetic nanoparticles during rewarming augments the recovery of hUCM-MSCs cryopreserved by vitrification[J]. Acta Biomaterialia, 2016, 33:264-274.
    [16]
    EISENBERG D P, BISCHOF J C, RABIN Y. Thermomechanical stress in cryopreservation via vitrification with nanoparticle heating as a stress-moderating effect[J]. Journal of Biomechanical Engineering, 2015, 138 (1):011010.
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    MANUCHEHRABADI N, GAO Z, ZHANG J, et al. Improved tissue cryopreservation using inductive heating of magnetic nanoparticles[J]. Science Translational Medicine, 2017, 9(379): eaah4586.
    [18]
    PAN J J, REN S, SEKAR P K, et al. Investigation of electromagnetic resonance rewarming enhanced by magnetic nanoparticles for cryopreservation[J]. Langmuir, 2019, 35(23):7560-7570.
    [19]
    XU Y, YU H, NIU Y, et al. Effects of superparamagnetic nanoparticles on nucleation and crystal growth in the vitrified Vs55 during warming[J]. Cryo Letters, 2016, 37(6):448-454.
    [20]
    于红梅, 胥义, 柳珂, 等. 磁纳米粒子对 Vs55 溶液反玻璃化等温结晶行为的影响[J]. 化工学报, 2017, 68(3):1262-1268.
    [21]
    SONG Y C, KHIRABADI B S, LIGHTFOOT F, et al. Vitreous cryopreservation maintains the function of vascular grafts[J]. Nature Biotechnology, 2000, 18(3):296-299.
    [22]
    BOURNE W M, NELSON L R. Human corneal studies with a vitrification solution containing dimethyl sulfoxide, formamide, and 1,2-propanediol.[J]. Cryobiology, 1994, 31(6):522-530.
    [23]
    KHEIRABADI B S, FAHY G M. Permanent life support by kidneys perfused with a vitrifiable (7.5 molar) cryoprotectant solution.[J]. Transplantation, 2000, 70(1):51-57.
    [24]
    BROCKBANK K G M. C-20: Tissue vitrification[J]. Cryobiology, 2014, 69(3):507.)
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    [1]
    华泽钊, 任禾盛. 低温生物医学技术[M]. 北京：科学出版社，1994.
    [2]
    FAHY G M, MACFARLANE D R, ANGELL C A, et al. Vitrification as an approach to cryopreservation[J]. Cryobiology, 1984, 21(4):407-426.
    [3]
    KULESHOVA L L, LOPATA A. Vitrification can be more favorable than slow cooling[J]. Fertility and Sterility, 2002, 78(3):449-454.
    [4]
    RALL W F, FAHY G M. Ice-free cryopreservation of mouse embryos at -196 ℃ by vitrification[J]. Nature, 1985, 313(6003):573-575.
    [5]
    HOU Y, LU C, DOU M, et al, Soft liquid metal nanoparticles achieve reduced crystal nucleation and ultrarapid rewarming for human bone marrow stromal cell and blood vessel cryopreservation[J]. Acta Biomaterialia1, 2020, 102: 403-415.
    [6]
    LI W J, ZHOU X L, LIU B L, et al. Effect of nanoparticles on the survival and development of vitrified porcine GV oocytes[J]. Cryo Letters, 2016, 37(6):401-405.
    [7]
    PAN J J, SHU Z Q, ZHAO G, et al. Towards uniform and fast rewarming for cryopreservation with electromagnetic resonance cavity: Numerical simulation and experimental investigation[J]. Applied Thermal Engineering, 2018, 140:787-798.
    [8]
    RING H L, GAO Z, SHARMA A, et al. Imaging the distribution of iron oxide nanoparticles in hypothermic perfused tissues[J]. Magnetic Resonance in Medicine, 2020, 83(5):1750-1759.
    [9]
    WANG T, ZHAO G, DENG Z S, et al. Theoretical investigation of a novel microwave antenna aided cryovial for rapid and uniform rewarming of frozen cryoprotective agent solutions[J]. Applied Thermal Engineering, 2015, 89:968-977.
    [10]
    ZHANG M, ZHAO G, GU N, et al. Applying nanotechnology to cryopreservation studies: Status and future[J]. Chinese Science Bulletin, 2019, 64(21):2180-2190.
    [11]
    ZHOU X, LI W, FANG L, et al. Hydroxyapatite nanoparticles improved survival rate of vitrified porcine oocytes and its mechanism[J]. Cryo Letters, 2015, 36(1):45-50.
    [12]
    MORNET S, VASSEUR S, GRASSET F, et al. Magnetic nanoparticle design for medical diagnosis and therapy[J]. Journal of Materials Chemistry, 2004, 14(14):2161.
    [13]
    WANG T, ZHAO G, LIANG X M, et al. Numerical simulation of the effect of superparamagnetic nanoparticles on microwave rewarming of cryopreserved tissues[J]. Cryobiology, 2014, 68(2):234-243.
    [14]
    ETHERIDGE M L, XU Y, ROTT L, et al. RF heating of magnetic nanoparticles improves the thawing of cryopreserved biomaterials[J]. Technology, 2014, 2(3):229-242.
    [15]
    WANG J, ZHAO G, ZHANG Z, et al. Magnetic induction heating of superparamagnetic nanoparticles during rewarming augments the recovery of hUCM-MSCs cryopreserved by vitrification[J]. Acta Biomaterialia, 2016, 33:264-274.
    [16]
    EISENBERG D P, BISCHOF J C, RABIN Y. Thermomechanical stress in cryopreservation via vitrification with nanoparticle heating as a stress-moderating effect[J]. Journal of Biomechanical Engineering, 2015, 138 (1):011010.
    [17]
    MANUCHEHRABADI N, GAO Z, ZHANG J, et al. Improved tissue cryopreservation using inductive heating of magnetic nanoparticles[J]. Science Translational Medicine, 2017, 9(379): eaah4586.
    [18]
    PAN J J, REN S, SEKAR P K, et al. Investigation of electromagnetic resonance rewarming enhanced by magnetic nanoparticles for cryopreservation[J]. Langmuir, 2019, 35(23):7560-7570.
    [19]
    XU Y, YU H, NIU Y, et al. Effects of superparamagnetic nanoparticles on nucleation and crystal growth in the vitrified Vs55 during warming[J]. Cryo Letters, 2016, 37(6):448-454.
    [20]
    于红梅, 胥义, 柳珂, 等. 磁纳米粒子对 Vs55 溶液反玻璃化等温结晶行为的影响[J]. 化工学报, 2017, 68(3):1262-1268.
    [21]
    SONG Y C, KHIRABADI B S, LIGHTFOOT F, et al. Vitreous cryopreservation maintains the function of vascular grafts[J]. Nature Biotechnology, 2000, 18(3):296-299.
    [22]
    BOURNE W M, NELSON L R. Human corneal studies with a vitrification solution containing dimethyl sulfoxide, formamide, and 1,2-propanediol.[J]. Cryobiology, 1994, 31(6):522-530.
    [23]
    KHEIRABADI B S, FAHY G M. Permanent life support by kidneys perfused with a vitrifiable (7.5 molar) cryoprotectant solution.[J]. Transplantation, 2000, 70(1):51-57.
    [24]
    BROCKBANK K G M. C-20: Tissue vitrification[J]. Cryobiology, 2014, 69(3):507.)
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