ISSN 0253-2778

CN 34-1054/N

open

Biocatalytic hydroxylation of vitamin D3 in ionic liquid biphasic systems

  • To explore the biocatalytic hydroxylation of vitamin D3 catalyzed by Bacillus cereus HDZJU1-11 in ionic liquid biphasic systems, a comparison was conducted of the substrate solubility and biocompatibility in 5 ionic liquids (PrMImPF6, BMImPF6, BMImNTf2, HMImPF6, and OMImNTf2) and the optimization of phase ratio, pH, and substrate concentration in ionic liquid biphasic systems was performed. BMImPF6, BMImNTf2, andOMImNTf2 were chosen to construct biphasic systems. In BMImPF6/water biphasic system, the optimal conversion condition is as follows: 0.5 mg/mL vitamin D3 and BMImPF6 (Vwater∶VIL=4∶1) are added after 12 h fermentation of Bacillus cereus, and the pH of water phase is 7.5. The concentration of 25-hydroxyvitamin D3 is increased to 0.38 mg/mL at 24 h fermentation. The optimal conversion conditions in ionic liquid biphasic systems are determined, which lays a foundation for expanding experiment and industrial applications.
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