Abstract: TD1 peptide, with the amino acid sequence of ACSSSPSKHCG, has been reported to have the potential ability to facilitate transdermal delivery of macromolecules such as insulin through skin. Here we report that TD1-GGS-eGFP (TGFP), or the N-terminal TD1 modified green fluorescent protein (GFP), compared to the unmodified GFP, is more capable of transporting through skin barrier in comparison with the unmodified GFP. This finding may provide a new way for transdermal drug delivery. It can also offer a new experimental methodology for the elucidation of the mechanism through which TD1 peptide facilitates transdermal delivery.