Action of glycine, lysine, tyrosine and methionine in Micromonospora echinospora

The promoters (amino acids) were screened out by the orthogonal design in the course of biosynthesis micronomicin from the mutant strain (Fzu-707) of Micromonospora echinospora. Certain amount of glycine, lysine, tyrosine and methionine was added to the original medium in a flask on the rotary shaker 220 r/min and incubated at 36 ℃. It was propitious to biosynthesize micronomicin in cells of Micromonospora echinospora. The fermentation time was shortened from 144 h to 104 h, the yield of micronomicin (u·mL-1) was increased about 20% compared with the control group and the production rate of micronomicin was increased about 60%.