Abstract: Chemical properties in a porous media have an important effect on solute transport. To study the solute transport affected by chemical reaction and flow rates, the properties of porous media were kept unchanged, and a case study of bimolecular reactive transport was conducted by using 1,2-naphthoquinone-4-sulfonic acid and aniline. Batch experiments and numerical simulations were conducted under different flow conditions, respectively. The main conclusions are as follows: ① The modified ADRE （advection-dispersion-reaction equation）model is feasible for bimolecular reactive transport with higher accuracy. The experimental results for reactive solute transport are in good agreement with those from the model of modified ADRE under the two different seepage velocities; the relative errors for the peak concentration of the product are 12% at 04 mL/s and 08% at 08 mL/s. ② The dispersion coefficient is identified for non-reactive transport by ADRE which can provide a technological reference to determine the dispersion coefficient for reactive transport. ③ With the increase of flow rates, the values of m decrease, while the values of β0 increase; in addition, the dispersion coefficients for reactive transport are slightly larger than that of non-reactive transport for the same solute at the same flow flux. Further mechanism study is needed.