Abstract: The main photosynthetic pigments utilized by plants for photosynthesis include chlorophyll A and chlorophyll B, while the primary photosensitizing pigments employed for physiological regulation are Pr and Pfr. These pigments exhibit absorption peaks predominantly in the blue, red, and near-infrared regions. Consequently, through a light-separation strategy, plants can selectively receive the required solar radiation while reflecting the remaining wavelengths for photovoltaic power generation. In this study, multilayer polymer films (MPFs) were employed as light filters to investigate their impact on plant growth. Comparative experiments were conducted to validate the efficacy of the light filtering system. Compared with those in the other groups, the environmental temperature and soil temperature of the plants in the MPF coverage group were lower, thereby alleviating the environmental stress faced by the plants. The fluorescence data confirmed that during noontime hours, the plants under the MPF presented increased photosynthetic efficiency, compensating for the reduced rates caused by insufficient solar radiation exposure. Furthermore, biomass analysis demonstrated MPF cover group had a 10.3% increase in plant yield compared with glass group. This investigation validates the feasibility of employing a light separation strategy in agriculture while providing robust empirical evidence supporting advancements in agricultural photovoltaics.