Abstract: This research aimed to design and fabricate a biocompatible dual-layer chitosan hydrogel adhesive patch with exceptional mechanical properties by employing a nanoadhesive strategy to assess its tissue adhesion performance. The design involves physical cross-linking to construct a robust chitosan hydrogel as a backing membrane, followed by in situ photocuring to create the adhesive hydrogel layer, resulting in an integrated chitosan hydrogel adhesive patch. To facilitate adhesion between the hydrogel patch and biological tissue, surface-activated silica nanoparticles serve as interfacial connectors, analogous to nanoglue, promoting binding of the hydrogel to the substrate. Characterization of the patch reveals an adhesive energy of 282 J/m² to biological tissues in vitro and a burst pressure of 450 mmHg (1 mmHg=0.133 kPa). The patch exhibits outstanding mechanical properties, with a tensile strength of 4.3 MPa, an elongation rate of 65%, and a fracture toughness of 3.82 kJ/m². Additionally, the nanohesion-based chitosan hydrogel adhesive patch is highly transparent and demonstrates excellent biocompatibility. It holds promise for applications in various biomedical fields, including tissue repair and drug delivery, thereby providing a robust material foundation for advancements in clinical surgery.